Welcome to the first on-line searchable database of primer sets useful for the detection of plant pathogenic fungi.

The idea was inspired by the article “THE THREE Ds OF PCR-BASED GENOMIC ANALYSIS OF PHYTOBACTERIA: Diversity,Detection, and Disease Diagnosis” by FJ Louws (1999), in which the authors listed a short but comprehensive collection of primer sets for the detection and identification of phytopathogenic bacteria.

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The collection of specific primers for plant pathogenic fungi was first published as a table on an italian specialized journal of national circulation [Ghignone S. and Migheli Q. (2001) Nuovi strumenti di diagnosi per i funghi fitopatogeni -tecniche basate sull’analisi degli acidi nucleici. Informatore Fitopatologico 51 (3):7-22], and later expanded and organized as a searchable database.

The database is currently curated by dr. Stefano Ghignone, researcher at Istituto per la Protezione Sostenibile delle Piante – Turin Unit (CNR) and dr. Quirico Migheli, Professor at University of Sassari, and is open to user contributions.

In February 2012, the site moved on a new server and its look has been updated. Follow this link to use the old site graphic interface.

While the database currently devoted to fungal organisms, in a future perspective the database may host primer sets specific to other phytopathogens, such as viruses or bacteria, including microbial herbicides and biological agents relevant in agroterrorism.

How to Cite

The Database of PCR Primers for Phytopathogenic Fungi
Stefano Ghignone, Quirico Migheli (2005)
European Journal of Plant Pathology 113 (1) p. 107-109
DOI: 10.1007/s10658-005-0652-y

SPPADBASE. The first on-line searchable database of PCR primers for phytopathogenic fungi
Stefano Ghignone, Quirico Migheli (2007)
Mycotechnology: Present Status And Future Prospects p. 173-180


Stefano Ghignone
Istituto per la Protezione Sostenibile delle Piante, SS Torino – CNR
c/o Dip. Scienze della Vita e Biologia dei Sistemi
V.le P.A. Mattioli, 25
I-10125 Turin
Phone: +39 011 6502927
Fax: +39 011 6705962
e-mail: stefano.ghignone[at]ipsp.cnr.it

Quirico Migheli
Dipartimento di Agraria
Università degli Studi di Sassari
Viale Italia 39
I-07100 Sassari
Phone: +39 079 229295/+39 320 9225548
Fax: +39 079 229316
E-mail: qmigheli[at]uniss.it

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