Primer set details

ORGANISM
Complete name : Phytophthora spp.
Database ID : 574
INTERNET RESOURCES
Google Wikispecies, the free species directory Wikipedia, The Free Encyclopeda
NCBI Taxonomy Browser CABI Index Fungorum CBS Anamorph/Teleomorph

PRIMER SET (5’→3′)
Forward Primer Select to Blast
Name : FITS_15Ph Sequence :
Reverse Primer
Name : RITS_279Ph Sequence :
Probe
Name : All_Phytophthora Sequence :
TARGET DNA
ITS region
PCR TECHNIQUE
Real Time PCR (TaqMan)
REFERENCE
Authors : Sikora, K., Verstappen, E., Mendes, O., Schoen, C., Ristaino, J., Bonants, P.
Year : 2012
Title : A Universal Microarray Detection Method for Identification of Multiple Phytophthora spp. Using Padlock Probes
Journal : Phytopathology
Volume : 102
Pages : 635-645
DOI: 10.1094/PHYTO-11-11-0309
Abstract : The genus Phytophthora consists of many species that cause important diseases in ornamental, agronomic, and forest ecosystems worldwide. Molecular methods have been developed for detection and identification of one or several species of Phytophthora in single or multiplex reactions. In this article, we describe a padlock probe (PLP)-based multiplex method of detection and identification for many Phytophthora spp. simultaneously. A generic TaqMan polymerase chain reaction assay, which detects all known Phytophthora spp., is conducted first, followed by a species-specific PLP ligation. A 96-well-based microarray platform with colorimetric readout is used to detect and identify the different Phytophthora spp. PLPs are long oligonucleotides containing target complementary sequence regions at both their 5′ and 3′ ends which can be ligated on the target into a circular molecule. The ligation is point mutation specific; therefore, closely related sequences can be differentiated. This circular molecule can then be detected on a microarray. We developed 23 PLPs to economically important Phytophthora spp. based upon internal transcribed spacer-1 sequence differences between individual Phytophthora spp. Tests on genomic DNA of many Phytophthora isolates and DNA from environmental samples showed the specificity and utility of PLPs for Phytophthora diagnostics.
SUBMITTER
Dr. Stefano Ghignone (Sistem Administrator)
stefano.ghignone@ipsp.cnr.it
Consiglio Nazionale delle Ricerche (CNR)
Istituto per la Protezione delle Piante, Sez. Torino
V.le P.A. Mattioli 25, I-10125 Turin
Italy
Record creation : 2012-08-31 Last record update : 2012-08-31

* Disclaimer *

No guarantee can be given for the correctness of the data presented.
Although the DNA sequences were reviewed for accuracy,
original pubblications should be consulted to verify their composition.

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