Primer set details

ORGANISM
Complete name : Plasmopara halstedii
Database ID : 573
INTERNET RESOURCES
Google Wikispecies, the free species directory Wikipedia, The Free Encyclopeda
NCBI Taxonomy Browser CABI Index Fungorum CBS Anamorph/Teleomorph

PRIMER SET (5’→3′)
Forward Primer Select to Blast
Name : qPHAL-F Sequence :
Reverse Primer
Name : qPHAL-R Sequence :
Probe
Name : qPHAL-P Sequence :
SOURCE SEQUENCE
GenBank acc no : AY035523
TARGET DNA (Amplicon size)
28S rDNA (94 bp)
PCR TECHNIQUE
Real Time PCR (TaqMan)
REFERENCE
Authors : Ioos, R., Fourrier, C., Wilson, V., Webb, K., Schereffer, J.-L., Tourvieille de Labrouhe, D.
Year : 2012
Title : An optimized duplex real-time PCR tool for sensitive detection of the quarantine oomycete Plasmopara Halstedii in sunflower seeds
Journal : Phytopathology
Volume : 102
Pages : 908-917
DOI: 10.1094/PHYTO-04-12-0068-R
Abstract : Plasmopara halstedii, the causal agent of downy mildew of sunflower, is an oomycete listed as a quarantine pathogen. This obligate parasite resides in a quiescent state in seeds of sunflower and can be spread from seed production areas to areas of crop production by international seed trade. To prevent the spread or the introduction of potentially new genotypes or fungicide-tolerant strains, an efficient method to detect P. halstedii in sunflower seed is required. This work reports the optimization of a real-time detection tool that targets the pathogen within sunflower seeds, and provides statistically validated data for that tool. The tool proved to be specific and inclusive, based on computer simulation and in vitro assessments, and could detect as few as 45 copies of target DNA. A fully optimized DNA extraction protocol was also developed starting from a sample of 1,000 sunflower seeds, and enabled the detection of <1 infected seed/1,000 seeds. To ensure reliability of the results, a set of controls was used systematically during the assays, including a plant-specific probe used in a duplex quantitative polymerase chain reaction that enabled the assessment of the quality of each DNA extract
SUBMITTER
Dr. Stefano Ghignone (Sistem Administrator)
stefano.ghignone@ipsp.cnr.it
Consiglio Nazionale delle Ricerche (CNR)
Istituto per la Protezione delle Piante, Sez. Torino
V.le P.A. Mattioli 25, I-10125 Turin
Italy
Record creation : 2012-08-31 Last record update : 2012-08-31

* Disclaimer *

No guarantee can be given for the correctness of the data presented.
Although the DNA sequences were reviewed for accuracy,
original pubblications should be consulted to verify their composition.

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