Primer set details

ORGANISM
Complete name : Phaeoacremonium aleophilum
Database ID : 572
Extra detection : Phaeomoniella chlamydospora
INTERNET RESOURCES
Google Wikispecies, the free species directory Wikipedia, The Free Encyclopeda
NCBI Taxonomy Browser CABI Index Fungorum CBS Anamorph/Teleomorph

PRIMER SET (5’→3′)
Forward Primer Select to Blast
Name : palFI1 Sequence :
Reverse Primer
Name : palRI1 Sequence :
Probe
Name : palI1 Sequence :
TARGET DNA (Amplicon size)
b-tubulin gene (63 bp)
PCR TECHNIQUE
Real Time PCR (TaqMan)
REFERENCE
Authors : Martín M.T., Cobos R., Martín L., López-Enrí­quez L.
Year : 2012
Title : Real-Time PCR Detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum
Journal : Applied and Environmental Microbiology
Volume : 78
Pages : 3985-3991
DOI: 10.1128/AEM.07360-11
Abstract : Phaeomoniella chlamydospora and Phaeoacremonium aleophilum are the two main fungal causal agents of Petri disease and esca. Both diseases cause significant economic losses to viticulturalists. Since no curative control measures are known, proactive defensive measures must be taken. An important aspect of current research is the development of sensitive and time-saving protocols for the detection and identification of these pathogens. Real-time PCR based on the amplification of specific sequences is now being used for the identification and quantification of many infective agents. The present work reports real-time PCR protocols for identification of P. chlamydospora and P. aleophilum. Specificity was demonstrated against purified DNA from 60 P. chlamydospora isolates or 61 P. aleophilum isolates, and no amplification was obtained with 54 nontarget DNAs. The limits of detection (i.e., DNA detectable in 95% of reactions) were around 100 fg for P. chlamydospora and 50 fg for P. aleophilum. Detection was specific and sensitive for P. chlamydospora and P. aleophilum. Spores of P. chlamydospora and P. aleophilum were detected without the need for DNA purification. The established protocols detected these fungi in wood samples after DNA purification. P. chlamydospora was detectable without DNA purification and isolation in 67% of reactions. The detection of these pathogens in wood samples has great potential for use in pathogen-free certification schemes.
SUBMITTER
Dr. Stefano Ghignone (Sistem Administrator)
stefano.ghignone@ipsp.cnr.it
Consiglio Nazionale delle Ricerche (CNR)
Istituto per la Protezione delle Piante, Sez. Torino
V.le P.A. Mattioli 25, I-10125 Turin
Italy
Record creation : 2012-08-31 Last record update : 2012-08-31

* Disclaimer *

No guarantee can be given for the correctness of the data presented.
Although the DNA sequences were reviewed for accuracy,
original pubblications should be consulted to verify their composition.

Back