Primer set details

ORGANISM
Complete name : Fusarium circinatum
Database ID : 484
INTERNET RESOURCES
Google Wikispecies, the free species directory Wikipedia, The Free Encyclopeda
NCBI Taxonomy Browser CABI Index Fungorum CBS Anamorph/Teleomorph

PRIMER SET (5’→3′)
Forward Primer Select to Blast
Name : CIRC1A Sequence :
Reverse Primer
Name : CIRC4A Sequence :
SOURCE SEQUENCE
GenBank acc no : AY249379
TARGET DNA (Amplicon size)
IGS region (360 bp)
PCR TECHNIQUE
Conventional PCR / Real Time PCR (SYBR Green I)
REFERENCE
Authors : Scheweigkofler W., O’Donell K., Garbelotto M.
Year : 2004
Title : Detection and quantification of airborne conidia of Fusarium circinatum, the causal agent of pine pitch canker, from two California sites by using a real-time PCR approach combined with a simple spore trapping method
Journal : Applied and Environmental Microbiology
Volume : 70
Pages : 3512-3520
DOI: 10.1128/AEM.70.6.3512-3520.2004
Abstract : Pinus radiata (Monterey pine), a tree native to coastal California and Mexico, is widely planted worldwide for timber production. A major threat to Monterey pine plantations is the fungal disease pine pitch canker, caused by Fusarium circinatum (Hypocreales). We present a novel trapping approach using filter paper in combination with a rapid molecular method to detect the presence of inoculum in the air. The assay is also useful for diagnosing the presence of the pathogen on plants. The test is based on the F. circinatum specific primer pair CIRC1A-CIRC4A, which amplifies a 360-bp DNA fragment in the intergenic spacer region of the nuclear ribosomal operon. Real-time PCR was used to calculate the number of fungal spores present in each reaction mixture by comparing the threshold cycle (Ct) of unknown spore samples to the Ct values of standards with known amounts of F. circinatum spores. The filter paper method allows prolonged and more sensitive spore sampling in the field compared to traditional traps using petri dishes filled with selective medium. A field test at two sites in coastal California infested with pine pitch canker was carried out during the summer and fall of 2002. Spore counts were in the range of ca. 1 x 10^3 to ca. 7 x 10^5/sqm, with the highest spore counts in the fall, suggesting a seasonal fluctuation.
SUBMITTER
Dr. Miguel Cambra
mcambra@aragon.es
Diputación General de Aragón
Centro de Protección Vegetal
Avda. Montañana, 930, 50059 Zaragoza
Spain
Record creation : 2008-01-03 Last record update : 2012-03-20

* Disclaimer *

No guarantee can be given for the correctness of the data presented.
Although the DNA sequences were reviewed for accuracy,
original pubblications should be consulted to verify their composition.

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