Primer set details

ORGANISM
Complete name : Fusarium spp.
Database ID : 313
INTERNET RESOURCES
Google Wikispecies, the free species directory Wikipedia, The Free Encyclopeda
NCBI Taxonomy Browser CABI Index Fungorum CBS Anamorph/Teleomorph

PRIMER SET (5’→3′)
Forward Primer Select to Blast
Name : ITS-Fu-f Sequence :
Reverse Primer
Name : ITS-Fu-r Sequence :
TARGET DNA (Amplicon size)
ITS region (389 bp)
PCR TECHNIQUE
Conventional PCR
REFERENCE
Authors : Abd-Elsalam K.A., Aly I.N., Abdel-Satar M.A., Khalil M.S., Verreet J.A.
Year : 2003
Title : PCR identification of Fusarium genus based on nuclear ribosomal-DNA sequence data
Journal : African Journal of Biotechnology
Volume : 2
Pages : 82-85
Abstract : We have developed two taxon-selective primers for quick identification of the Fusarium genus. These primers, ITS-Fu-f and ITS-Fu-r were designed by comparing the aligned sequences of internal transcribed spacer regions (ITS) of a range of Fusarium species. The primers showed good specificity for the genus Fusarium, and the approximately 389-bp product was amplified exclusively. PCR sensitivity ranged from 100 fg to 10 ng for DNA extracted from Fusarium oxysporum mycelium. No amplification products were detected with PCR of DNA from Rhizoctonia solani and Macrophomina phaseolina isolates using these primers. The assay is useful for rapid identification of Fusarium spp.cultures. The application of these PCR methods for early diagnosis of the seedling and wilt disease of cotton needs to be studied further.
SUBMITTER
Dr. Kamel Abd-Elsalam
abdelsalamka@gmail.com
Agricultural Research Center
Plant Pathology Research Institute – Molecular Markers
9-Gamaa St., Giza
Egypt
Record creation : 2004-09-13 Last record update : 2004-09-13

* Disclaimer *

No guarantee can be given for the correctness of the data presented.
Although the DNA sequences were reviewed for accuracy,
original pubblications should be consulted to verify their composition.

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