The Next NGS Challenge Conference is a joint event of the EU COST Action SeqAhead, the EMBnet, the International Society of Computational Biology ISCB and the 7FP Project STATegra that aims to become a dedicated meeting on cutting-edge Next Generation Sequencing applications. The goal of this Conference is to bring together computational biologist and bioinformaticians . . . → Read More: The Next NGS Challenge Conference
Phytophthora sojae is a devastating pathogen that causes soybean Phytophthora root rot. This study reports the development of a loop-mediated isothermal amplification (LAMP) assay targeting the A3aPro element for visual detection of P. sojae. The A3aPro-LAMP assay efficiently amplified the target element in < 80 min at 64 °C and was evaluated for specificity and sensitivity. The . . . → Read More: Development of a loop-mediated isothermal amplification assay for detection of Phytophthora sojae
Background Fungal load quantification is a critical component of fungal community analyses. Limitation of current approaches for quantifying the fungal component in the human microbiome suggests the need for new broad-coverage techniques. Methods We analyzed 2,085 18S rRNA gene sequences from the SILVA database for assay design. We generated and quantified plasmid . . . → Read More: FungiQuant: A broad-coverage fungal quantitative real-time PCR assay
[ Eiken GENOME SITE ] – The principle of LAMP method.
“LAMP” which stands for Loop-mediated Isothermal Amplification is a simple, rapid, specific and cost-effective nucleic acid amplification method solely developed by Eiken Chemical Co., Ltd. It is characterized by the use of 4 different primers specifically designed to recognize 6 distinct regions on . . . → Read More: The principle of LAMP method
The combined data set of the acl1 and tef-1α gene sequences of 61 fungal strains assigned to Fusarium tricinctum, Fusarium avenaceum, Fusarium acuminatum, Fusarium arthrosporioides, Fusarium flocciferum and Fusarium torulosum were used to study the phylogenetic relations between taxa. F. tricinctum, F. acuminatum and F. avenaceum formed distinct clades. Members of the F. . . . → Read More: ATP citrate lyase 1 (acl1) gene-based loop-mediated amplification assay for the detection of the Fusarium tricinctum species complex in pure cultures and in cereal samples